Composite
Part:BBa_K3002123:Design
Designed by: Marlene Schlosser Group: iGEM19_TU_Kaiserslautern (2019-10-21)
Level 1 – MHETase + cCA + sp20-His
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1327
Illegal PstI site found at 1651
Illegal PstI site found at 1994
Illegal PstI site found at 2804 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 249
Illegal PstI site found at 1327
Illegal PstI site found at 1651
Illegal PstI site found at 1994
Illegal PstI site found at 2804
Illegal NotI site found at 1662 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2572
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1327
Illegal PstI site found at 1651
Illegal PstI site found at 1994
Illegal PstI site found at 2804 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1327
Illegal PstI site found at 1651
Illegal PstI site found at 1994
Illegal PstI site found at 2804
Illegal NgoMIV site found at 1260
Illegal NgoMIV site found at 1721
Illegal NgoMIV site found at 1751
Illegal NgoMIV site found at 2066
Illegal NgoMIV site found at 2084
Illegal NgoMIV site found at 2120
Illegal NgoMIV site found at 2763 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.
Source
This part is a combination of the parts BBa_K3002027 (PAR-promoter), BBa_K3002006 (RPL23-Terminator), BBa_K3002037 (CDS of MHETase), BBa_K3002007 (cCA), BBa_K3002018 (spSP20-His-tag), BBa_K3002302 (connector B1-B2), BBa_K3002303 (connector B2-B3), BBa_K3002304 (connector B4-B5) and BBa_K3002305 (connector B5-B6).